Chimeric Epitope Vaccine from Multistage Antigens for Lymphatic Filariasis.
Identifieur interne : 002023 ( Main/Exploration ); précédent : 002022; suivant : 002024Chimeric Epitope Vaccine from Multistage Antigens for Lymphatic Filariasis.
Auteurs : G. Anugraha [Inde] ; J. Madhumathi [Inde] ; P R Prince [Inde] ; P J Jeya Prita [Inde] ; V K Khatri [Inde] ; N P Amdare [Inde] ; M V R. Reddy [Inde] ; P. Kaliraj [Inde]Source :
- Scandinavian journal of immunology [ 1365-3083 ] ; 2015.
Descripteurs français
- KwdFr :
- Animaux, Anticorps antihelminthe (immunologie), Anticorps antiprotozoaires (sang), Antigènes d'helminthe (immunologie), Brugia malayi (immunologie), Brugia malayi (pathogénicité), Filariose lymphatique (), Gerbillinae, Humains, Modèles animaux de maladie humaine, Mâle, Protéines d'helminthes (immunologie), Protéines de fusion recombinantes (administration et posologie), Protéines de fusion recombinantes (immunologie), Protéines recombinantes (immunologie), Souris, Thiorédoxines (immunologie), Transglutaminases (immunologie), Vaccination, Vaccins antiprotozoaires (administration et posologie), Vaccins antiprotozoaires (immunologie), Wuchereria bancrofti (pathogénicité), Épitopes immunodominants (administration et posologie), Épitopes immunodominants (immunologie).
- MESH :
- administration et posologie : Protéines de fusion recombinantes, Vaccins antiprotozoaires, Épitopes immunodominants.
- immunologie : Anticorps antihelminthe, Antigènes d'helminthe, Brugia malayi, Protéines d'helminthes, Protéines de fusion recombinantes, Protéines recombinantes, Thiorédoxines, Transglutaminases, Vaccins antiprotozoaires, Épitopes immunodominants.
- pathogénicité : Brugia malayi, Wuchereria bancrofti.
- sang : Anticorps antiprotozoaires.
- Animaux, Filariose lymphatique, Gerbillinae, Humains, Modèles animaux de maladie humaine, Mâle, Souris, Vaccination.
English descriptors
- KwdEn :
- Animals, Antibodies, Helminth (immunology), Antibodies, Protozoan (blood), Antigens, Helminth (immunology), Brugia malayi (immunology), Brugia malayi (pathogenicity), Disease Models, Animal, Elephantiasis, Filarial (prevention & control), Gerbillinae, Helminth Proteins (immunology), Humans, Immunodominant Epitopes (administration & dosage), Immunodominant Epitopes (immunology), Male, Mice, Protozoan Vaccines (administration & dosage), Protozoan Vaccines (immunology), Recombinant Fusion Proteins (administration & dosage), Recombinant Fusion Proteins (immunology), Recombinant Proteins (immunology), Thioredoxins (immunology), Transglutaminases (immunology), Vaccination, Wuchereria bancrofti (pathogenicity).
- MESH :
- chemical , administration & dosage : Immunodominant Epitopes, Protozoan Vaccines, Recombinant Fusion Proteins.
- chemical , blood : Antibodies, Protozoan.
- chemical , immunology : Antibodies, Helminth, Antigens, Helminth, Helminth Proteins, Immunodominant Epitopes, Protozoan Vaccines, Recombinant Fusion Proteins, Recombinant Proteins, Thioredoxins, Transglutaminases.
- immunology : Brugia malayi.
- pathogenicity : Brugia malayi, Wuchereria bancrofti.
- prevention & control : Elephantiasis, Filarial.
- Animals, Disease Models, Animal, Gerbillinae, Humans, Male, Mice, Vaccination.
Abstract
Lymphatic filariasis, a mosquito-borne parasitic disease, affects more than 120 million people worldwide. Vaccination for filariasis by targeting different stages of the parasite will be a boon to the existing MDA efforts of WHO which required repeated administration of the drug to reduce the infection level and sustained transmission. Onset of a filaria-specific immune response achieved through antigen vaccines can act synergistically with these drugs to enhance the parasite killing. Multi-epitope vaccine approach has been proved to be successful against several parasitic diseases as it overcomes the limitations associated with the whole antigen vaccines. Earlier results from our group suggested the protective efficacy of multi-epitope vaccine comprising two immunodominant epitopes from Brugia malayi antioxidant thioredoxin (TRX), several epitopes from transglutaminase (TGA) and abundant larval transcript-2 (ALT-2). In this study, the prophylactic efficacy of the filarial epitope protein (FEP), a chimera of selective epitopes identified from our earlier study, was tested in a murine model (jird) of filariasis with L3 larvae. FEP conferred a significantly (P < 0.0001) high protection (69.5%) over the control in jirds. We also observed that the multi-epitope recombinant construct (FEP) induces multiple types of protective immune responses, thus ensuring the successful elimination of the parasite; this poses FEP as a potential vaccine candidate.
DOI: 10.1111/sji.12340
PubMed: 26179420
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream PubMed, to step Corpus: 000D70
- to stream PubMed, to step Curation: 000D70
- to stream PubMed, to step Checkpoint: 000D70
- to stream Ncbi, to step Merge: 007622
- to stream Ncbi, to step Curation: 007622
- to stream Ncbi, to step Checkpoint: 007622
- to stream Main, to step Merge: 002026
- to stream Main, to step Curation: 002023
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Chimeric Epitope Vaccine from Multistage Antigens for Lymphatic Filariasis.</title><author><name sortKey="Anugraha, G" sort="Anugraha, G" uniqKey="Anugraha G" first="G" last="Anugraha">G. Anugraha</name><affiliation wicri:level="1"><nlm:affiliation>Centre for Biotechnology, Anna University, Chennai, India.</nlm:affiliation><country xml:lang="fr">Inde</country><wicri:regionArea>Centre for Biotechnology, Anna University, Chennai</wicri:regionArea><wicri:noRegion>Chennai</wicri:noRegion></affiliation></author><author><name sortKey="Madhumathi, J" sort="Madhumathi, J" uniqKey="Madhumathi J" first="J" last="Madhumathi">J. Madhumathi</name><affiliation wicri:level="1"><nlm:affiliation>Centre for Biotechnology, Anna University, Chennai, India.</nlm:affiliation><country xml:lang="fr">Inde</country><wicri:regionArea>Centre for Biotechnology, Anna University, Chennai</wicri:regionArea><wicri:noRegion>Chennai</wicri:noRegion></affiliation></author><author><name sortKey="Prince, P R" sort="Prince, P R" uniqKey="Prince P" first="P R" last="Prince">P R Prince</name><affiliation wicri:level="1"><nlm:affiliation>Centre for Biotechnology, Anna University, Chennai, India.</nlm:affiliation><country xml:lang="fr">Inde</country><wicri:regionArea>Centre for Biotechnology, Anna University, Chennai</wicri:regionArea><wicri:noRegion>Chennai</wicri:noRegion></affiliation></author><author><name sortKey="Prita, P J Jeya" sort="Prita, P J Jeya" uniqKey="Prita P" first="P J Jeya" last="Prita">P J Jeya Prita</name><affiliation wicri:level="1"><nlm:affiliation>Centre for Biotechnology, Anna University, Chennai, India.</nlm:affiliation><country xml:lang="fr">Inde</country><wicri:regionArea>Centre for Biotechnology, Anna University, Chennai</wicri:regionArea><wicri:noRegion>Chennai</wicri:noRegion></affiliation></author><author><name sortKey="Khatri, V K" sort="Khatri, V K" uniqKey="Khatri V" first="V K" last="Khatri">V K Khatri</name><affiliation wicri:level="1"><nlm:affiliation>Department of Biochemistry, Mahatma Gandhi Institute of Medical Sciences, Sevagram, India.</nlm:affiliation><country xml:lang="fr">Inde</country><wicri:regionArea>Department of Biochemistry, Mahatma Gandhi Institute of Medical Sciences, Sevagram</wicri:regionArea><wicri:noRegion>Sevagram</wicri:noRegion></affiliation></author><author><name sortKey="Amdare, N P" sort="Amdare, N P" uniqKey="Amdare N" first="N P" last="Amdare">N P Amdare</name><affiliation wicri:level="1"><nlm:affiliation>Department of Biochemistry, Mahatma Gandhi Institute of Medical Sciences, Sevagram, India.</nlm:affiliation><country xml:lang="fr">Inde</country><wicri:regionArea>Department of Biochemistry, Mahatma Gandhi Institute of Medical Sciences, Sevagram</wicri:regionArea><wicri:noRegion>Sevagram</wicri:noRegion></affiliation></author><author><name sortKey="Reddy, M V R" sort="Reddy, M V R" uniqKey="Reddy M" first="M V R" last="Reddy">M V R. Reddy</name><affiliation wicri:level="1"><nlm:affiliation>Department of Biochemistry, Mahatma Gandhi Institute of Medical Sciences, Sevagram, India.</nlm:affiliation><country xml:lang="fr">Inde</country><wicri:regionArea>Department of Biochemistry, Mahatma Gandhi Institute of Medical Sciences, Sevagram</wicri:regionArea><wicri:noRegion>Sevagram</wicri:noRegion></affiliation></author><author><name sortKey="Kaliraj, P" sort="Kaliraj, P" uniqKey="Kaliraj P" first="P" last="Kaliraj">P. Kaliraj</name><affiliation wicri:level="1"><nlm:affiliation>Centre for Biotechnology, Anna University, Chennai, India.</nlm:affiliation><country xml:lang="fr">Inde</country><wicri:regionArea>Centre for Biotechnology, Anna University, Chennai</wicri:regionArea><wicri:noRegion>Chennai</wicri:noRegion></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2015">2015</date><idno type="RBID">pubmed:26179420</idno><idno type="pmid">26179420</idno><idno type="doi">10.1111/sji.12340</idno><idno type="wicri:Area/PubMed/Corpus">000D70</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000D70</idno><idno type="wicri:Area/PubMed/Curation">000D70</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">000D70</idno><idno type="wicri:Area/PubMed/Checkpoint">000D70</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">000D70</idno><idno type="wicri:Area/Ncbi/Merge">007622</idno><idno type="wicri:Area/Ncbi/Curation">007622</idno><idno type="wicri:Area/Ncbi/Checkpoint">007622</idno><idno type="wicri:Area/Main/Merge">002026</idno><idno type="wicri:Area/Main/Curation">002023</idno><idno type="wicri:Area/Main/Exploration">002023</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Chimeric Epitope Vaccine from Multistage Antigens for Lymphatic Filariasis.</title><author><name sortKey="Anugraha, G" sort="Anugraha, G" uniqKey="Anugraha G" first="G" last="Anugraha">G. Anugraha</name><affiliation wicri:level="1"><nlm:affiliation>Centre for Biotechnology, Anna University, Chennai, India.</nlm:affiliation><country xml:lang="fr">Inde</country><wicri:regionArea>Centre for Biotechnology, Anna University, Chennai</wicri:regionArea><wicri:noRegion>Chennai</wicri:noRegion></affiliation></author><author><name sortKey="Madhumathi, J" sort="Madhumathi, J" uniqKey="Madhumathi J" first="J" last="Madhumathi">J. Madhumathi</name><affiliation wicri:level="1"><nlm:affiliation>Centre for Biotechnology, Anna University, Chennai, India.</nlm:affiliation><country xml:lang="fr">Inde</country><wicri:regionArea>Centre for Biotechnology, Anna University, Chennai</wicri:regionArea><wicri:noRegion>Chennai</wicri:noRegion></affiliation></author><author><name sortKey="Prince, P R" sort="Prince, P R" uniqKey="Prince P" first="P R" last="Prince">P R Prince</name><affiliation wicri:level="1"><nlm:affiliation>Centre for Biotechnology, Anna University, Chennai, India.</nlm:affiliation><country xml:lang="fr">Inde</country><wicri:regionArea>Centre for Biotechnology, Anna University, Chennai</wicri:regionArea><wicri:noRegion>Chennai</wicri:noRegion></affiliation></author><author><name sortKey="Prita, P J Jeya" sort="Prita, P J Jeya" uniqKey="Prita P" first="P J Jeya" last="Prita">P J Jeya Prita</name><affiliation wicri:level="1"><nlm:affiliation>Centre for Biotechnology, Anna University, Chennai, India.</nlm:affiliation><country xml:lang="fr">Inde</country><wicri:regionArea>Centre for Biotechnology, Anna University, Chennai</wicri:regionArea><wicri:noRegion>Chennai</wicri:noRegion></affiliation></author><author><name sortKey="Khatri, V K" sort="Khatri, V K" uniqKey="Khatri V" first="V K" last="Khatri">V K Khatri</name><affiliation wicri:level="1"><nlm:affiliation>Department of Biochemistry, Mahatma Gandhi Institute of Medical Sciences, Sevagram, India.</nlm:affiliation><country xml:lang="fr">Inde</country><wicri:regionArea>Department of Biochemistry, Mahatma Gandhi Institute of Medical Sciences, Sevagram</wicri:regionArea><wicri:noRegion>Sevagram</wicri:noRegion></affiliation></author><author><name sortKey="Amdare, N P" sort="Amdare, N P" uniqKey="Amdare N" first="N P" last="Amdare">N P Amdare</name><affiliation wicri:level="1"><nlm:affiliation>Department of Biochemistry, Mahatma Gandhi Institute of Medical Sciences, Sevagram, India.</nlm:affiliation><country xml:lang="fr">Inde</country><wicri:regionArea>Department of Biochemistry, Mahatma Gandhi Institute of Medical Sciences, Sevagram</wicri:regionArea><wicri:noRegion>Sevagram</wicri:noRegion></affiliation></author><author><name sortKey="Reddy, M V R" sort="Reddy, M V R" uniqKey="Reddy M" first="M V R" last="Reddy">M V R. Reddy</name><affiliation wicri:level="1"><nlm:affiliation>Department of Biochemistry, Mahatma Gandhi Institute of Medical Sciences, Sevagram, India.</nlm:affiliation><country xml:lang="fr">Inde</country><wicri:regionArea>Department of Biochemistry, Mahatma Gandhi Institute of Medical Sciences, Sevagram</wicri:regionArea><wicri:noRegion>Sevagram</wicri:noRegion></affiliation></author><author><name sortKey="Kaliraj, P" sort="Kaliraj, P" uniqKey="Kaliraj P" first="P" last="Kaliraj">P. Kaliraj</name><affiliation wicri:level="1"><nlm:affiliation>Centre for Biotechnology, Anna University, Chennai, India.</nlm:affiliation><country xml:lang="fr">Inde</country><wicri:regionArea>Centre for Biotechnology, Anna University, Chennai</wicri:regionArea><wicri:noRegion>Chennai</wicri:noRegion></affiliation></author></analytic><series><title level="j">Scandinavian journal of immunology</title><idno type="eISSN">1365-3083</idno><imprint><date when="2015" type="published">2015</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Antibodies, Helminth (immunology)</term><term>Antibodies, Protozoan (blood)</term><term>Antigens, Helminth (immunology)</term><term>Brugia malayi (immunology)</term><term>Brugia malayi (pathogenicity)</term><term>Disease Models, Animal</term><term>Elephantiasis, Filarial (prevention & control)</term><term>Gerbillinae</term><term>Helminth Proteins (immunology)</term><term>Humans</term><term>Immunodominant Epitopes (administration & dosage)</term><term>Immunodominant Epitopes (immunology)</term><term>Male</term><term>Mice</term><term>Protozoan Vaccines (administration & dosage)</term><term>Protozoan Vaccines (immunology)</term><term>Recombinant Fusion Proteins (administration & dosage)</term><term>Recombinant Fusion Proteins (immunology)</term><term>Recombinant Proteins (immunology)</term><term>Thioredoxins (immunology)</term><term>Transglutaminases (immunology)</term><term>Vaccination</term><term>Wuchereria bancrofti (pathogenicity)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term><term>Anticorps antihelminthe (immunologie)</term><term>Anticorps antiprotozoaires (sang)</term><term>Antigènes d'helminthe (immunologie)</term><term>Brugia malayi (immunologie)</term><term>Brugia malayi (pathogénicité)</term><term>Filariose lymphatique ()</term><term>Gerbillinae</term><term>Humains</term><term>Modèles animaux de maladie humaine</term><term>Mâle</term><term>Protéines d'helminthes (immunologie)</term><term>Protéines de fusion recombinantes (administration et posologie)</term><term>Protéines de fusion recombinantes (immunologie)</term><term>Protéines recombinantes (immunologie)</term><term>Souris</term><term>Thiorédoxines (immunologie)</term><term>Transglutaminases (immunologie)</term><term>Vaccination</term><term>Vaccins antiprotozoaires (administration et posologie)</term><term>Vaccins antiprotozoaires (immunologie)</term><term>Wuchereria bancrofti (pathogénicité)</term><term>Épitopes immunodominants (administration et posologie)</term><term>Épitopes immunodominants (immunologie)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="administration & dosage" xml:lang="en"><term>Immunodominant Epitopes</term><term>Protozoan Vaccines</term><term>Recombinant Fusion Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="blood" xml:lang="en"><term>Antibodies, Protozoan</term></keywords><keywords scheme="MESH" type="chemical" qualifier="immunology" xml:lang="en"><term>Antibodies, Helminth</term><term>Antigens, Helminth</term><term>Helminth Proteins</term><term>Immunodominant Epitopes</term><term>Protozoan Vaccines</term><term>Recombinant Fusion Proteins</term><term>Recombinant Proteins</term><term>Thioredoxins</term><term>Transglutaminases</term></keywords><keywords scheme="MESH" qualifier="administration et posologie" xml:lang="fr"><term>Protéines de fusion recombinantes</term><term>Vaccins antiprotozoaires</term><term>Épitopes immunodominants</term></keywords><keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Anticorps antihelminthe</term><term>Antigènes d'helminthe</term><term>Brugia malayi</term><term>Protéines d'helminthes</term><term>Protéines de fusion recombinantes</term><term>Protéines recombinantes</term><term>Thiorédoxines</term><term>Transglutaminases</term><term>Vaccins antiprotozoaires</term><term>Épitopes immunodominants</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Brugia malayi</term></keywords><keywords scheme="MESH" qualifier="pathogenicity" xml:lang="en"><term>Brugia malayi</term><term>Wuchereria bancrofti</term></keywords><keywords scheme="MESH" qualifier="pathogénicité" xml:lang="fr"><term>Brugia malayi</term><term>Wuchereria bancrofti</term></keywords><keywords scheme="MESH" qualifier="prevention & control" xml:lang="en"><term>Elephantiasis, Filarial</term></keywords><keywords scheme="MESH" qualifier="sang" xml:lang="fr"><term>Anticorps antiprotozoaires</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Disease Models, Animal</term><term>Gerbillinae</term><term>Humans</term><term>Male</term><term>Mice</term><term>Vaccination</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Filariose lymphatique</term><term>Gerbillinae</term><term>Humains</term><term>Modèles animaux de maladie humaine</term><term>Mâle</term><term>Souris</term><term>Vaccination</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Lymphatic filariasis, a mosquito-borne parasitic disease, affects more than 120 million people worldwide. Vaccination for filariasis by targeting different stages of the parasite will be a boon to the existing MDA efforts of WHO which required repeated administration of the drug to reduce the infection level and sustained transmission. Onset of a filaria-specific immune response achieved through antigen vaccines can act synergistically with these drugs to enhance the parasite killing. Multi-epitope vaccine approach has been proved to be successful against several parasitic diseases as it overcomes the limitations associated with the whole antigen vaccines. Earlier results from our group suggested the protective efficacy of multi-epitope vaccine comprising two immunodominant epitopes from Brugia malayi antioxidant thioredoxin (TRX), several epitopes from transglutaminase (TGA) and abundant larval transcript-2 (ALT-2). In this study, the prophylactic efficacy of the filarial epitope protein (FEP), a chimera of selective epitopes identified from our earlier study, was tested in a murine model (jird) of filariasis with L3 larvae. FEP conferred a significantly (P < 0.0001) high protection (69.5%) over the control in jirds. We also observed that the multi-epitope recombinant construct (FEP) induces multiple types of protective immune responses, thus ensuring the successful elimination of the parasite; this poses FEP as a potential vaccine candidate.</div></front></TEI><affiliations><list><country><li>Inde</li></country></list><tree><country name="Inde"><noRegion><name sortKey="Anugraha, G" sort="Anugraha, G" uniqKey="Anugraha G" first="G" last="Anugraha">G. Anugraha</name></noRegion><name sortKey="Amdare, N P" sort="Amdare, N P" uniqKey="Amdare N" first="N P" last="Amdare">N P Amdare</name><name sortKey="Kaliraj, P" sort="Kaliraj, P" uniqKey="Kaliraj P" first="P" last="Kaliraj">P. Kaliraj</name><name sortKey="Khatri, V K" sort="Khatri, V K" uniqKey="Khatri V" first="V K" last="Khatri">V K Khatri</name><name sortKey="Madhumathi, J" sort="Madhumathi, J" uniqKey="Madhumathi J" first="J" last="Madhumathi">J. Madhumathi</name><name sortKey="Prince, P R" sort="Prince, P R" uniqKey="Prince P" first="P R" last="Prince">P R Prince</name><name sortKey="Prita, P J Jeya" sort="Prita, P J Jeya" uniqKey="Prita P" first="P J Jeya" last="Prita">P J Jeya Prita</name><name sortKey="Reddy, M V R" sort="Reddy, M V R" uniqKey="Reddy M" first="M V R" last="Reddy">M V R. Reddy</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Sante/explor/LymphedemaV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 002023 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 002023 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/Sante
|area= LymphedemaV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= pubmed:26179420
|texte= Chimeric Epitope Vaccine from Multistage Antigens for Lymphatic Filariasis.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:26179420" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
| NlmPubMed2Wicri -a LymphedemaV1
| This area was generated with Dilib version V0.6.31. |  |